Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique

Rapid Detection and Quantification of Rhynchosporium secalis in Barley Using a Polymerase Chain Reaction

PCR primers for diagnosis of Rhynchosporium secalis in seed samples of barley were developed. For the quantification of the pathogen in seed samples a real-time PCR with SYBR Green approach was used. Amounts from 1.8 to 419.1 pg of R. secalis DNA per 100 ng of total DNA were detected in 18 samples of barley seeds contaminated by R. secalis in field conditions. The correctness of this quantitati...

Transcriptome Analysis of the Barley-Rhynchosporium secalis Interaction

Leaf scald caused by the infection of Rhynchosporium secalis, is a worldwide crop disease resulting in significant loss of barley yield. In this study, a systematic sequencing of expressed sequence tags (ESTs) was chosen to obtain a global picture of the assembly of genes involved in pathogenesis. To identify a large number of plant ESTs, which are induced at different time points, an amplified...

Detection of tetracycline resistance genes in bacteria isolated from fish farms using polymerase chain reaction

Five common tetracycline resistance genes tet(A), tet(B), tet(M), tet(O) and tet(S) were studied by polymerase chain reaction in 100 bacteria isolated from Iranian fish farms. In the antibiogram test most of the bacteria were either intermediately or completely resistant to tetracycline. Nine isolates out of 46 Aeromonas spp. contained eithe...

Detection of the eaeA Gene in Escherichia coli Isolated from Broiler Chickens by Polymerase Chain Reaction

The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in Escherichia coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs (lung, liver and spleen) were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. Escherichiacoliwas isolated from ...

Detection of Babesia ovis Using Polymerase Chain Reaction.